Journal: Journal of Cellular and Molecular Medicine

Article Title: Byakangelicin protects against carbon tetrachloride–induced liver injury and fibrosis in mice

doi: 10.1111/jcmm.15493

Figure Lengend Snippet: Byakangelicin inhibits 4‐HNE–induced hepatocyte apoptosis by inhibiting ASK1/JNK pathway. A, Detection cytotoxicity of byakangelicin in liver stellate cell line LX2 using MTT. B and C, Different time and concentration gradients to detect 4‐HNE–induced hepatocyte HepG2 apoptosis protein full‐length PARP, PARP, cleaved caspase‐3, caspase‐3 and internal reference protein β‐tubulin expression with densitometry. D and E, Protein full‐length PARP, PARP, cleaved caspase‐3, caspase‐3, JNK, P‐JNK, ASK‐1 and P‐ASK‐1 were also detected and quantified using Western blot analyses. F, Immunofluorescence by using antibody against caspase‐3. G, Apoptosis detection by using flow cytometry. For the statistics of each panel in this figure, # P < .05, ## P < .01, ### P < .001; * P < .05, ** P < .01, *** P < .001, n = 3

Article Snippet: We selected HepG2 as human hepatocyte cell line., The HepG2 cells were cultured in DMEM (Solarbio Technology) with 10% foetal bovine serum (FBS; Excell Bio).

Techniques: Concentration Assay, Expressing, Western Blot, Immunofluorescence, Flow Cytometry

Journal: Journal of Cellular and Molecular Medicine

Article Title: Byakangelicin protects against carbon tetrachloride–induced liver injury and fibrosis in mice

doi: 10.1111/jcmm.15493

Figure Lengend Snippet: Mechanism diagram of byakangelicin attenuating the progression of liver fibrosis. byakangelicin inhibits the proliferation and activation of hepatic stellate cell by inhibiting the TGF/Smad3 signalling pathway and PDGF‐ERK, AKT and Stat3 signalling. In addition, byakangelicin could inhibit 4‐HNE–induced apoptosis and reduce death signalling through the ASK/JNK pathway in hepatocytes

Article Snippet: We selected HepG2 as human hepatocyte cell line., The HepG2 cells were cultured in DMEM (Solarbio Technology) with 10% foetal bovine serum (FBS; Excell Bio).

Techniques: Activation Assay

Journal: Molecular Cancer Therapeutics

Article Title: Preclinical Profile of BYON3521 Predicts an Effective and Safe MET Antibody–Drug Conjugate

doi: 10.1158/1535-7163.MCT-22-0596

Figure Lengend Snippet: Competition experiments with rhHGF and MET ECD. A, BLI experiment to assess competition of rhHGF for MET ECD binding of SYD2884. Presented are the binding curve of SYD2884 visualized with an HRP-conjugated anti-hIgG (red line, solid squares) and rhHGF visualized using a polyclonal anti-HGF antibody (blue line, solid triangles). The detectable amount of SYD2884 binding to the recombinant human MET ECD, immobilized on the surface of the sensor tip, decreases whereas at the same time the detectable amount of rhHGF increases. The data are presented as the correlation between the measured signals versus the molar excess of fully activated rhHGF (left graph) and pro-HGF (right graph). B, Cell viability of EBC-1 cells treated with increasing concentrations of BYON3521 or nonbinding isotype control ADC in the presence or absence of 1 μg/mL MET ECD, either administered together directly, or after 30-minute preincubation of BYON3521 or isotype control ADC and MET ECD (Premix). (Approximate MW of BYON3521 is 149 kD, of SY2884 144 kD, and MET ECD 140 kD).

Article Snippet: To evaluate the potential interference of HGF with cellular binding, cells were preincubated with 320 ng/mL recombinant human (rh)HGF (30 minutes at 4°C; Sino Biological; mixture of pro-HGF and activated HGF) prior to incubation with antibody or ADC.

Techniques: Binding Assay, Recombinant

Journal: Journal of Clinical Medicine

Article Title: A Novel Bioengineered Functional Motor Unit Platform to Study Neuromuscular Interaction

doi: 10.3390/jcm9103238

Figure Lengend Snippet: Complete growth media for skeletal muscle cell proliferation.

Article Snippet: Recombinant human hepatocyte growth factor (HGF) from Sino Biological Inc. (Beijing, China) , 2.5 ng/mL , 10463-HNAS.

Techniques: Concentration Assay, Modification, Recombinant

Journal: Scientific Reports

Article Title: Autophagy inhibition enhances antifibrotic potential of placental mesenchymal stem cells of fetal origin via regulating TGF-β1 mediated protein degradation of HGF

doi: 10.1038/s41598-025-97054-8

Figure Lengend Snippet: Effects of HGF regulated by autophagy on antifibrotic function of fPMSCs. The conditioned medium from sh-NC and sh-ATG5 infection of fPMSCs were infected with sh-NC and sh-ATG5 lentivirus and pretreated with 20 ng/mL TGF-β1 in order to prepare conditioned medium, then which was conducted to culture MRC-5 cells stimulated with 2.5 ng/mL TGF-β1 for 24 h. 500 ng/mL recombinant human HGF (rhHGF) and 1 μg/mL HGF neutralization antibody (naHGF) was added into conditioned medium respectively to interrupt the function of HGF. Protein levels of Collagen I, α-SMA and CTGF were detected by western blotting. Densitometric analyses of Collagen I, α-SMA and CTGF. Representative data from three independent experiments are shown. * P < 0.05.

Article Snippet: A coculture system was treated with 500 ng/mL recombinant human HGF (Proteintech, China) and 1 μg/mL HGF neutralization antibody (R&D Systems, USA) to verify the antifibrotic function of HGF derived from fPMSCs on MRC-5 cells.

Techniques: Infection, Recombinant, Neutralization, Western Blot

Journal: Experimental Biology and Medicine

Article Title: Mesenchymal stem cells alleviate hypoxia-induced oxidative stress and enhance the pro-survival pathways in porcine islets

doi: 10.1177/1535370219844472

Figure Lengend Snippet: IL-6 plays a role in MSCs' protection. (a) ELISA quantification of IL-6 and HGF in MSC-CM. (b) The recombinant human IL-6 (rIL-6) decreased the hypoxia-induced death of NICCs. rIL-6 suppressed total ROS accumulation (c) and augmented protein phosphorylation (d) under hypoxic conditions. nor: normoxia; hypo: hypoxia; CM: conditioned medium. Values are means ± SD. **P < 0.01, ***P < 0.001. (A color version of this figure is available in the online journal.)

Article Snippet: Human IL6 ELISA Kit (Proteintech, USA) and human hepatocyte growth factor (HGF) ELISA kit (CUSABIO, China) were used to detect the concentration of IL-6 and HGF in MSC-CM, respectively.

Techniques: Enzyme-linked Immunosorbent Assay, Recombinant, Phospho-proteomics